GGCT protein (His tag) (80R-1645)
Purified recombinant Human GGCT protein
|Synonyms||MGC3077, Ggc protein, CRF21 protein, Gamma-glutamylcyclotransferase protein, C7orf24 protein, FLJ11717 protein, GCTG protein|
Coomassie Blue stained SDS-PAGE of GGCT protein (His tag) (80R-1645)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-188 amino acids: MGSSHHHHHH SSGLVPRGSH MANSGCKDVT GPDEESFLYF AYGSNLLTER IHLRNPSAAF FCVARLQDFK LDFGNSQGKT SQTWHGGIAT IFQSPGDEVW GVVWKMNKSN LNSLDEQEGV KSGMYVVIEV KVATQEGKEI TCRSYLMTNY ESAPPSPQYK KIICMGAKEN GLPLEYQEKL KAIEPNDYTG KVSEEIEDII KKGETQTL|
|Purity||> 95% pure|
|Molecular Weight||23.2 kDa (208aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||GGCT, also known as gamma-glutamylcyclotransferase, belongs to the family of transferases, specifically the aminoacyltransferases. This protein catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides and may play a significant role in glutathione homeostasis. GGCT induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Recombinant human GGCT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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