Glucose 6 Phosphate Isomerase antibody (10R-2136)

Mouse monoclonal Glucose 6 Phosphate Isomerase antibody

Synonyms Monoclonal Glucose 6 Phosphate Isomerase antibody, Anti-Glucose 6 Phosphate Isomerase antibody, GPI antibody, Phosphoglucose isomerase antibody, Glucose 6 Phosphate Isomerase,, -, antibody, - antibody
Cross Reactivity Human
Applications ELISA, IF, WB
Immunogen Glucose 6 Phosphate Isomerase antibody was raised in Mouse using a purified recombinant fragment of human GPI expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using Glucose 6 Phosphate Isomerase antibody (10R-2136) (Glucose 6 Phosphate Isomerase antibody)

Confocal immunofluorescence analysis of L-02 cells using GPI antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 1B7D7
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Glucose 6 Phosphate Isomerase belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways and it is an enzyme that catalyzes the conversion of glucose-6-phosphate into fructose 6-phosphate in the second step of glycolysis. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in GPI are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

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Images

  • Immunofluorescent staining using Western Blot analysis using Glucose 6 Phosphate Isomerase antibody (10R-2136) (Glucose 6 Phosphate Isomerase antibody) | Confocal immunofluorescence analysis of L-02 cells using GPI antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using Glucose 6 Phosphate Isomerase antibody (10R-2136) | Western Blot showing GPI antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GPI cDNA (2).
  • Western Blot analysis using Glucose 6 Phosphate Isomerase antibody (20198) | Western Blot showing using GPI antibody used against HepG2 (1), SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).
  • Immunohistochemical staining using Glucose 6 Phosphate Isomerase antibody (10R-2136) | Immunohistochemical analysis of paraffin-embedded human Kidney tissues using GPI antibody.

Availability: In stock

Price: $375.00
Size: 100 ul
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