Goat anti Human IgG (Fab'2) (FITC) (43C-CJ0119)

Goat anti Human IgG secondary antibody (Fab'2) (FITC)

Synonyms Goat anti Human Immunoglobulin G secondary antibody (Fab'2) (FITC)
Specificity Human IgG (Fab2)
Cross Reactivity To be determined by end-user
Applications ELISA, FC, IF
Immunogen Goat anti-human IgG (Fab'2) (FITC) was raised in goat using human IgG F(ab')2 fragment as the immunogen.

Images

Western blot analysis using Goat anti Human IgG (Fab'2) (FITC) (43C-CJ0119)

Western blot of FITC conjugated Goat F(ab’)2 Anti-Human IgG F(ab’)2. Lane 1: Human Fab2. Lane 2: None. Load: 50 ng per lane. None. FITC Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 kDa, 25 kDa for Human IgG F(ab’)2..

Specifications

Host Goat
Method of Purification Goat anti Human IgG (Fab'2) (FITC) was purified by affinity chromatography.
Tag/Conjugate FITC
Form & Buffer Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fab region, (fragment, antigen binding), is the binding region of the antibody.

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Images

  • Western blot analysis using Goat anti Human IgG (Fab'2) (FITC) (43C-CJ0119) | Western blot of FITC conjugated Goat F(ab’)2 Anti-Human IgG F(ab’)2. Lane 1: Human Fab2. Lane 2: None. Load: 50 ng per lane. None. FITC Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 kDa, 25 kDa for Human IgG F(ab’)2..

Availability: In stock

Price: $275.00
Size: 1 mg
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