Goat anti Human IgG (Fc) (Agarose Conjugated) (43R-IG016AS)

Goat anti Human IgG secondary antibody (Fc) (Agarose Conjugated)

Synonyms Goat anti Human Immunoglobulin G secondary antibody (Fc) (Agarose Conjugated)
Specificity Fc fragment of human IgG
Cross Reactivity Specific for the Fc fragment of human IgG when testedagainst purified human IgA, IgG, IgM, Fc, and kappaand lambda light chains. No cross reactivity with mouse orrat IgG is observed by Ouchterlony Double Diffusion(ODD), prior to agarose bead coupling.
Applications IP, ODD
Immunogen Goat anti human IgG (Fc) (Agarose Conjugated) was raised in goat using human IgG Fc fragment as the immunogen.

Specifications

Host Goat
Tag/Conjugate Agarose
Form & Buffer Supplied as a 1:1 suspension of antibody/resin in 0.02M K3PO4,pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
Concentration 2 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user
Bioactivity 1 ml Goat Anti-Human IgG (Fc fragment)-Agarose binds 3 mg human IgG.

Storage & Safety

Storage Store at 4 deg C. DO NOT FREEZE.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Affinity isolated antibody obtained from goat anti-human IgG antiserum following removal of all goat serum proteins, including immunoglobulins which do not specifically bind to the Fc fragment of human IgG, by immunospecific purification. The antibody preparation is solid phase adsorbed with mouse and rat IgG's to ensure minimal cross reactivity in cell or tissue preparations. The isolated antibody is coupled to cyanogen bromide-activated agarose at approximately 4 mg/ml wt/vol of antibody/resin. The antibody-resin is then mixed 1:1 in a PBS suspension giving a 2 mg/ml final concentration.

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Availability: In stock

Price: $325.00
Size: 2 ml
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