Goat anti Rabbit IgG (H + L) (rhodamine) (43C-CB1103)

Goat anti Rabbit IgG (H + L) secondary antibody (Rhodamine)

Synonyms Goat anti Rabbit Immunoglobulin G (H + L) secondary antibody (Rhodamine)
Specificity Rabbit IgG (H + L)
Cross Reactivity To be determined by end-user
Applications ELISA, FC, IF
Immunogen Goat anti-rabbit IgG (H+L) (Rhodamine) was raised in goat using rabbit IgG, whole molecule as the immunogen.

Images

Western blot analysis using Goat anti Rabbit IgG (H + L) (rhodamine) (43C-CB1103)

Western Blot of Rabbit IgG Rhodamine Conjugated Secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Rhodamine Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..

Specifications

Host Goat
Method of Purification Goat anti Rabbit IgG (H + L) (Rhodamine) was purified by affinity chromatography.
Tag/Conjugate Rhodamine
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

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Images

  • Western blot analysis using Goat anti Rabbit IgG (H + L) (rhodamine) (43C-CB1103) | Western Blot of Rabbit IgG Rhodamine Conjugated Secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Rhodamine Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG..
  • Dot Blot analysis using Goat anti Rabbit IgG (H + L) (rhodamine) (43C-CB1103) | Dot Blot of Rhodamine Conjugated Goat-anti-Rabbit IgG. Antigen: Rabbit IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Goat-a-Rabbit IgG secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.

Availability: In stock

Price: $175.00
Size: 2 mg
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