Goat anti Rabbit IgG (HRP) (43C-CB1141)

Goat anti Rabbit IgG secondary antibody (HRP)

Synonyms Goat anti Rabbit Immunoglobulin G secondary antibody (HRP)
Specificity Rabbit IgG
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Goat anti-rabbit IgG (HRP) was raised in goat using rabbit IgG F(c) fragment as the immunogen.

Images

Western blot analysis using Goat anti Rabbit IgG (HRP) (43C-CB1141)

Western blot of Peroxidase conjugated Rabbit IgG F(c) secondary antibody. Lane 1: Rabbit IgG F(c). Lane 2: None. Load: 50 ng per lane. None. Peroxidase Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Rabbit IgG F(c)..

Specifications

Host Goat
Method of Purification Goat anti Rabbit IgG (HRP) was purified by affinity chromatography.
Tag/Conjugate HRP
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2 with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamicin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:110,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:20,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

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Images

  • Western blot analysis using Goat anti Rabbit IgG (HRP) (43C-CB1141) | Western blot of Peroxidase conjugated Rabbit IgG F(c) secondary antibody. Lane 1: Rabbit IgG F(c). Lane 2: None. Load: 50 ng per lane. None. Peroxidase Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Rabbit IgG F(c)..

Availability: In stock

Price: $295.00
Size: 2 mg
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