GOT2 antibody (10R-1966)

Mouse monoclonal GOT2 antibody

Synonyms Monoclonal GOT2 antibody, Anti-GOT2 antibody, Glutamic-Oxaloacetic Transaminase 2 Mitochondrial antibody, Aspartate Aminotransferase 2 antibody, FLJ40994 antibody, kat4 antibody, kativ antibody, mitaat antibody, GOT2, GOT 2, GOT-2, GOT 2 antibody, GOT-2 antibody
Cross Reactivity Human, Mouse, Rat, Monkey
Applications ELISA, IF, WB
Immunogen GOT2 antibody was raised in Mouse using a purified recombinant fragment of human GOT2 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using GOT2 antibody (10R-1966) (GOT2 antibody)

Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 3E9
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

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Images

  • Immunofluorescent staining using Western Blot analysis using GOT2 antibody (10R-1966) (GOT2 antibody) | Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using GOT2 antibody (10R-1966) | Western Blot showing GOT2 antibody used against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.

Availability: In stock

Price: $345.00
Size: 100 ul
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