GOT2 antibody (10R-1966)
Mouse monoclonal GOT2 antibody
|Synonyms||Monoclonal GOT2 antibody, Anti-GOT2 antibody, Glutamic-Oxaloacetic Transaminase 2 Mitochondrial antibody, Aspartate Aminotransferase 2 antibody, FLJ40994 antibody, kat4 antibody, kativ antibody, mitaat antibody, GOT2, GOT 2, GOT-2, GOT 2 antibody, GOT-2 antibody|
|Cross Reactivity||Human, Mouse, Rat, Monkey|
|Applications||ELISA, IF, WB|
|Immunogen||GOT2 antibody was raised in Mouse using a purified recombinant fragment of human GOT2 expressed in E. coli as the immunogen.|
Immunofluorescent staining using Western Blot analysis using GOT2 antibody (10R-1966) (GOT2 antibody)
Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.|
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