GPC4 antibody (10R-1168)

Mouse monoclonal GPC4 antibody

Synonyms Monoclonal GPC4 antibody, Anti-GPC4 antibody, GPC -4 antibody, glypican proteoglycan 4 antibody, GPC4, GPC 4 antibody, glypican 4 antibody, GPC -4, GPC 4, K-glypican antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IF, WB
Immunogen GPC4 antibody was raised in mouse using recombinant human GPC4 (401-529aa) purified from E. coli as the immunogen.

Images

Flow Cytometryanalysis of GPC4 antibody (10R-1168)

Flow cytometry analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Specifications

Host Mouse
Clone AT51E3
Isotype IgG1 kappa
Source Anti-human GPC4 antibody, clone AT4A3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GPC4 protein.
Method of Purification GPC4 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:500, WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The glypican (Gpc) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the stage of intiation of neural tube closure, suggesting that glypican-4 is unlikely to play an essential role in convergent extension in the mouse.

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Images

  • Flow Cytometryanalysis of GPC4 antibody (10R-1168) | Flow cytometry analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
  • Western Blot analysis of GPC4 antibody (10R-1168) | Cell lysates of Mouse kidney lysates (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GPC4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conj ugated to HRP and an ECL detection system.
  • Immunofluorescent staining using GPC4 antibody (10R-1168) | Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human GPC4 antibody (1:500) with Texas Red (Red).

Availability: In stock

Price: $265.00
Size: 100 ul
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