GPT2 protein (His tag) (80R-2618)
Purified recombinant Human GPT2 protein (His tag)
|Synonyms||GPT 2, AAT-2 protein, ALT2 protein, AAT 2 protein, GPT-2 protein, AAT2 protein, ALT 2 protein, GPT 2 protein, ALT-2 protein, GPT2, Alanine aminotransferase 2 protein, GPT-2|
SDS-PAGE analysis of GPT2 protein (His tag) (80R-2618)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-523 aa: MGSSHHHHHH SSGLVPRGSH MGSMQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA|
|Method of Purification||GPT2 protein was purified using Protein G affinity chromatography|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 7.5) containing 30% glycerol 0.2M NaCl, 2mM DTT|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney. Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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