GPX1(U49C) protein (His tag) (80R-2026)

Recombinant human GPX1(U49C) protein (His tag)

Synonyms GPX1(U49C), GPX(U49C)-1 protein, GPX(U49C)-1, GPX(U49C) 1, GSHPX1 protein, GPX(U49C) 1 protein, Glutathione peroxidase 1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of GPX1(U49C) protein (His tag) (80R-2026)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-203 amino acids: MGSSHHHHHH SSGLVPRGSH MCAARLAAAA AAAQSVYAFS ARPLAGGEPV SLGSLRGKVL LIENVASLCG TTVRDYTQMN ELQRRLGPRG LVVLGFPCNQ FGHQENAKNE EILNSLKYVR PGGGFEPNFM LFEKCEVNGA GAHPLFAFLR EALPAPSDDA TALMTDPKLI TWSPVCRNDV AWNFEKFLVG PDGVPLRRYS RRFQTIDIEP DIEALLSQGP SCA
Expression System E.coli
Purity > 90% pure
Molecular Weight 24.2 kDa (223aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 100mM NaCl
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations GPX1(U49C) protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance GPX1 (Glutathione peroxidase 1) belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. The GPX1 is part of the enzymatic antioxidant defence, preventing oxidative damage to DNA, proteins and lipids by detoxifying hydrogen and lipid peroxides that may contribute to prostate cancer development. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX1(U49C) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

References
Add a Paper

Dhimant Desaia, Naveen Kaushalb, Ujjawal H. Gandhib, Ryan J. Arnerb, Christopher D'Souzae, Gang Chenf, Hema Vuntab, Karam El-Bayoumyg, Shantu Amina, K. Sandeep Prabhu

Synthesis and evaluation of the anti-inflammatory properties of selenium-derivatives of celecoxib

Chemico-Biological Interactions

Volume: 188 Issue: 3 Page: 446-456 DOI: 10.1016/j.cbi.2010.09.021

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Images

  • Coomassie Blue stained SDS-PAGE of GPX1(U49C) protein (His tag) (80R-2026) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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