GRHPR protein (His tag) (80R-1341)
Purified recombinant Human GRHPR protein
|Synonyms||OTTHUMP00000046131 protein, GLYD protein, PH2 protein, Glyoxylate reductase/hydroxypyruvate reductase glycerate 2 dehydrogenase protein, GLXR protein, Glyoxylate reductase/hydroxypyruvate reductase protein, PH 2 protein, , Primary hyperoxaluria type 2 protein|
Coomassie Blue stained SDS-PAGE of GRHPR protein (His tag) (80R-1341)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-328 amino acids: MGSSHHHHHH SSGLVPRGSH MRPVRLMKVF VTRRIPAEGR VALARAADCE VEQWDSDEPI PAKELERGVA GAHGLLCLLS DHVDKRILDA AGANLKVIST MSVGIDHLAL DEIKKRGIRV GYTPDVLTDT TAELAVSLLL TTCRRLPEAI EEVKNGGWTS WKPLWLCGYG LTQSTVGIIG LGRIGQAIAR RLKPFGVQRF LYTGRQPRPE EAAEFQAEFV STPELAAQSD FIVVACSLTP ATEGLCNKDF FQKMKETAVF INISRGDVVN QDDLYQALAS GKIAAAGLDV TSPEPLPTNH PLLTLKNCVI LPHIGSATHR TRNTMSLLAA NNLLAGLRGE PMPSELKL|
|Purity||> 95% pure|
|Molecular Weight||37.8 kDa (348aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||GRHPR, also known as GLXR, is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family of proteins. Localizing to the cytosol, GRHPR is ubiquitously expressed with highest expression levels found in liver. The protein has widespread tissue expression and has a role in metabolism. GRHPR is an enzyme with hydroxypyruvate reductase, glyoxylate reductase, and D-glycerate dehydrogenase enzymatic activities. Recombinant human GRHPR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques|
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