GRLF1 antibody (10R-1537)

Mouse monoclonal GRLF1 antibody

Synonyms Monoclonal GRLF1 antibody, Anti-GRLF1 antibody, GRLF 1, GRLF1, Glucocorticoid receptor DNA binding factor 1 antibody, p190RhoGAP antibody, GRLF-1, MGC10745 antibody, P190-A antibody, GRLF 1 antibody, GRF-1 antibody, P190A antibody, GRLF-1 antibody, KIAA1722 antibody
Specificity Human GRLF1
Cross Reactivity Human. Other species not studied
Applications Dot Blot
Immunogen GRLF1 antibody was raised in mouse using recombinant Human Glucocorticoid Receptor Dna Binding Factor 1


Western Blot analysis of immunized recombinant protein using GRLF1 antibody (10R-1537)


Host Mouse
Clone 2389D3a
Isotype IgG1
Method of Purification GRLF1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer GRLF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deducedf rom the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif.

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  • Western Blot analysis of immunized recombinant protein using GRLF1 antibody (10R-1537)

Availability: In stock

Price: $190.00
Size: 50 ug
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