GrpE protein (30R-1113)

Purified recombinant E.coli GrpE protein

Synonyms GrpE protein, HSP-70 cofactor protein, Heat shock protein B25.3 protein, HSP24 protein, HSP 70 cofactor.
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of GrpE protein (30R-1113)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
Expression System E.coli
Purity > 90% pure
Molecular Weight 21.8 kDa (197aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris, pH 8.0, containing 100mM NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance GrpE ,co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of GrpE protein (30R-1113) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 100 ug
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