GSK3B antibody (10R-1862)

Mouse monoclonal GSK3B antibody

Synonyms Monoclonal GSK3B antibody, Anti-GSK3B antibody, GSK3B, GSKB-3 antibody, GSKB-3, GSK3 beta antibody, GSKB 3 antibody, GSKB 3, Glycogen synthase kinase 3 beta antibody
Cross Reactivity Human, Rat, Mouse, Monkey
Applications ELISA, IF, IHC, WB
Immunogen GSK3B antibody was raised in Mouse using a purified recombinant fragment of human GSK3B expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using GSK3B antibody (10R-1862) (GSK3B antibody)

FC analysis of Hela cells using GSK3B antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 3D10
Isotype IgG2a
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Glycogen synthase kinase 3 beta is a serine/threonine kinase involved in insulin, growth factor and Wnt signaling. Glycogen synthase kinase 3 beta (GSk3beta) is a molecular regulator of cell fate in the central nervous system and a target of the phosphatidylinositol 3-kinase (PI3k) pathway. It is an enzyme with a diverse number of actions in intracellular signaling systems, regulating neuronal plasticity, gene expression and cell survival.

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Images

  • Flow Cytometric assay using Western Blot analysis using GSK3B antibody (10R-1862) (GSK3B antibody) | FC analysis of Hela cells using GSK3B antibody (green) and negative control (purple).
  • Western Blot analysis using GSK3B antibody (10R-1862) | Western Blot showing GSK3B antibody used against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.
  • Immunohistochemical staining using GSK3B antibody (10R-1862) | Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B antibody with DAB staining.
  • Immunofluorescent staining using Western Blot analysis using GSK3B antibody (10R-1862) (GSK3B antibody) | Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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