GST antibody (10R-1798)
Mouse monoclonal GST antibody
|Synonyms||Monoclonal GST antibody, Anti-GST antibody, GST antibody, Sj26 antigen antibody, Glutathione S-Transferase antibody, SjGST antibody, GST 26 antibody|
|Immunogen||GST antibody was raised in Mouse using purified fusion protein with GST protein tag expressed in E. coli as the immunogen.|
Western Blot analysis using GST antibody (10R-1798)
Western Blot showing GST antibody used against various fusion protein with GST tag.
|Form & Buffer||Purified antibody supplied in PBS containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins. Monoclonal antibody reacting specifically with GST may be useful in various immunotechnique, to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors|
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