GTF2B protein (His tag) (80R-1236)

Purified recombinant Human GTF2B protein

Synonyms TF2B protein, GTFB-2 protein, TFIIB protein, GTF2B, S300 II protein, General transcription factor IIB General transcription factor TFIIB protein, GTF2B protein, GTFB 2, Transcription initiation factor IIB., TF IIB protein, GTFB-2, GTFB 2 protein, RNA polymerase II transcription factor IIB protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of GTF2B protein (His tag) (80R-1236)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-316 amino acids: MGSSHHHHHH SSGLVPRGSH MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF PTDFKFDTPV DKLPQL
Expression System E.coli
Purity > 90% pure
Molecular Weight 36.9 kDa (336aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1 M NaCl.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance GTF2B, also known as transcription factor II B, is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. It localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. The protein serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. It is involved in the selection of the transcription start site. Recombinant GTF2B protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of GTF2B protein (His tag) (80R-1236) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $220.00
Size: 100 ug
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