GTF3C2 antibody (10R-1421)

Mouse monoclonal GTF3C2 antibody

Synonyms Monoclonal GTF3C2 antibody, Anti-GTF3C2 antibody, TFIIIC-BETA antibody, GTFC2-3 antibody, GTF3C2, GTFC2 3, GTFC2-3, TFIIIC110 antibody, KIAA0011 antibody, General transcription factor 3C polypeptide 2 antibody, GTFC2 3 antibody
Specificity Human GTF3C2
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen GTF3C2 antibody was raised in mouse using recombinant Human General Transcription Factor Iiic, Polypeptide 2, Beta 110Kda (Gtf3C2)

Images

Immunofluorescent staining using GTF3C2 antibody (10R-1421)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-GTF3C2 antibody.

Specifications

Host Mouse
Clone 2395C2a
Isotype IgG1
Method of Purification GTF3C2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer GTF3C2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance GTF3C2 is required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.

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Images

  • Immunofluorescent staining using GTF3C2 antibody (10R-1421) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-GTF3C2 antibody.
  • Western Blot analysis of immunized recombinant protein using GTF3C2 antibody (10R-1421)

Availability: In stock

Price: $190.00
Size: 50 ug
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