H3F3A protein (His tag) (80R-2788)

Purified recombinant H3F3A protein (His tag)

Synonyms Histone H3.3 protein, H3.3A protein, H3F3 protein, H3F3A
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of H3F3A protein (His tag) (80R-2788)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-136 aa: MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA
Expression System E.coli
Purity > 90% pure
Molecular Weight > 90% by SDS - PAGE
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A is a replication-independent member of the histone H3 family. Recombinant human H3F3A protein, fused to His-tag at N-terminus, was expressed in E.coli.

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Images

  • SDS-PAGE analysis of H3F3A protein (His tag) (80R-2788) | Figure annotation denotes ug of protein loaded and % gel used.
  • SDS-PAGE analysis of H3F3A protein (His tag) (80R-2788) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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