HA tag antibody (10R-8404)

Mouse monoclonal HA tag antibody

Synonyms Monoclonal HA tag antibody, Anti-HA tag antibody, HA epitope tag HA tag HAHA1 HA2 hemagglutinin antibody
Applications ELISA, ICC, IP, WB
Immunogen HA tag antibody was raised in Mouse using a recombinant human HA-tag protein purified E.coli as the immunogen

Images

Western Blot analysis using HA tag antibody (10R-8404)

Specifications

Host Mouse
Clone LF7H5
Isotype IgG1 kappa
Method of Purification HA tag antibody was purified by ammonium sulfate precipitation
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50%glycerol

Usage & Assay Information

Usage Recommendations WB: 1:10000-50000, IP: 1-2 ul/ml cell lysates, ICC: 1:500

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage. The expression vectors are used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. Short pieces of welldefined peptides (Poly-His, Flag-epitope or cmyc epitope or HA-tag) or small proteins (bacterial GST, MBP) is often cloned along with the target gene. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors. It has been successfully used in a variety of applications, including Western blotting, Immunoprecipitation, and immunofluorescence studies.

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Images

  • Western Blot analysis using HA tag antibody (10R-8404) |

Availability: In stock

Price: $295.00
Size: 100 ul
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