HAO1 protein (His tag) (80R-1929)
Purified recombinant HAO1 protein
|Synonyms||HAO1, Hydroxyacid oxidase 1 protein, HAO 1 protein, HAO-1 protein, HAO-1, GOX1 protein, GOX (glycolate oxidase) protein, HAO 1, HAOX1 protein|
|Assay Information||HAO1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.|
Coomassie Blue stained SDS-PAGE of HAO1 protein (His tag) (80R-1929)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-370 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIV AKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQ GEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI|
|Purity||> 95% pure|
|Molecular Weight||45.0 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 20% glycerol and 0.5M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidase (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on 2-carbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant human HAO1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
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