HAS1 antibody (10R-2222)

Mouse monoclonal HAS1 antibody

Synonyms Monoclonal HAS1 antibody, Anti-HAS1 antibody, HA synthase 1 antibody, HAS 1, HA antibody, HAS-1, HAS-1 antibody, HAS 1 antibody, HAS1, Hyaluronate synthase 1 antibody, Hyaluronan synthase 1 antibody, Hyaluronic acid antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen HAS1 antibody was raised in Mouse using a purified recombinant fragment of human HAS1 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using HAS1 antibody (10R-2222) (HAS1 antibody)

Immunofluorescence analysis of U251 cells using HAS1 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 3E10
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance HAS1 is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HAS1 is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate.

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Images

  • Immunofluorescent staining using Western Blot analysis using HAS1 antibody (10R-2222) (HAS1 antibody) | Immunofluorescence analysis of U251 cells using HAS1 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using HAS1 antibody (10R-2222) | Western Blot showing HAS1 antibody used against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)

Availability: In stock

Price: $345.00
Size: 100 ul
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