HD antibody (10R-1577)

Mouse monoclonal HD antibody

Synonyms Monoclonal HD antibody, Anti-HD antibody, Huntington disease antibody, HTT antibody, IT15 antibody
Specificity Human HD
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen HD antibody was raised in mouse using recombinant Human Huntingtin (Huntington Disease) (Hd)

Images

Immunofluorescent staining using HD antibody (10R-1577)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-HD antibody.

Specifications

Host Mouse
Clone 2401C1a
Isotype IgG2b
Method of Purification HD antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer HD antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Huntingtin is a disease gene linked to Huntington?s disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues.

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Images

  • Immunofluorescent staining using HD antibody (10R-1577) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-HD antibody.
  • Western Blot analysis of immunized recombinant protein using HD antibody (10R-1577)

Availability: In stock

Price: $190.00
Size: 50 ug
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