HDDC3 protein (His tag) (80R-2020)

Recombinant human HDDC3 protein (His tag)

Synonyms HDDC 3 protein, Guanosine-3' protein, HDDC3, HDDC-3, HDDC 3, 5'-bis(diphosphate)-pyrophosphohydrolase MESH1 protein, MESH1 protein, ppGpp protein, HDDC-3 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of HDDC3 protein (His tag) (80R-2020)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-140 amino acids: MGSSHHHHHH SSGLVPRGSH MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL HDTVEDTDTT LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL ADKLYNLRDL NRCTPEVKIQ
Expression System E.coli
Purity > 90% pure
Molecular Weight 17.9 kDa (160aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations HDDC3 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance HDDC3, also known as guanosine-3',5'-bis(diphosphate)-pyrophosphohydrolase MESH1, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn(2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3',5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria. Recombinant human HDDC3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of HDDC3 protein (His tag) (80R-2020) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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