HeLa Cell Lysate (H2O2 stimulated) (35R-1900)

HeLa Cell Lysate (H2O2 stimulated) for use in the research laboratory

Synonyms H2O2 stimulated HeLa cells
Applications WB
Assay Information A 100 ul vial ofunstimulated cellsis supplied in addition to the 100 ul vial of stimulated cells.

Specifications

Source Human epithelial cell line
Form & Buffer Cells were cultured for 30 minutes in the presence of 1mM H2O2 (simulatedcells only)and lysed in Lysis buffer (20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstati
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Immunoblot analysis.

General Information

Biological Significance

References
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Availability: In stock

Price: $95.00
Size: 100 ul
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