HIF1a protein (His tag) (80R-1492)
Purified recombinant Human HIF1a protein
|Synonyms||HIF1a, HIFa-1 protein, PASD8 protein, HIFa 1 protein, HIFa 1, BHLHE78 protein, Hypoxia-inducible factor 1-alpha, HIFa-1, MOP1 protein|
Coomassie Blue stained SDS-PAGE of HIF1a protein (His tag) (80R-1492)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-85 amino acids: MGSSHHHHHH SSGLVPRGSH MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMK|
|Purity||> 80% pure|
|Molecular Weight||11.8 kDa (105aa), confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris buffer, pH 8.0, containing 20% glycerol, 1mM DTT, 0.2M NaCl and 1mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1 beta subunit and one of three subunits (Hif-1 alpha, Hif-2 alpha or Hif-3 alpha). The activation of Hif-1 alpha is closely associated with a variety of tumors and oncogenic pathways. Hif-1 alpha consists of DNA binding domain(DBD domain), dimerization domain and C-terminal regulatory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains. Recombinant human Hif-1 alpha (1-85) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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