HIF1A protein (30R-3212)

Purified recombinant HIF1A protein

Synonyms HIF 1 alpha protein, HIF1 alpha protein, ARNT interacting protein, Basic helix loop helix PAS MOP1 protein, Class E basic helix loop helix 78 protein, bHLHe78 protein, Member of PAS 1 protein, PAS domain containing 8 protein, Hypoxia inducible factor 1 alpha protein, HIF 1A protein, HIF-1A protein
Species Human
Protein Type Recombinant
Applications ELISA, SDS-PAGE, WB


Western blot analysis using HIF1A protein (30R-3212)


Expression System E.coli
Purity > 90% pure
Molecular Weight 34 kDa
Form & Buffer Supplied in liquid form in 20mM Tris-Hcl, 0.5MNaCl, 10% glycerin (pH 8.0) and 200 mM Imidazole

Storage & Safety

Storage Store at 4 deg C in a working aliquot for 1 week. For long term storage, aliquot and freeze at -20 to -80 deg C, avoid repeat freeze/thaw cycles

General Information

Biological Significance HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300.

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  • Western blot analysis using HIF1A protein (30R-3212)

Availability: In stock

Price: $210.00
Size: 50 ug
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