His Tag antibody (HRP) (60R-1099)
Rabbit polyclonal His Tag antibody (HRP)
|Synonyms||Polyclonal His Tag antibody, Anti-His Tag antibody, 6XHis antibody, Polyhistidine Tag antibody, HIS6 Tag antibody, 6-His antibody, HHHHHH antibody, 6-His antibody|
|Applications||ELISA, ICC, WB|
|Immunogen||His Tag antibody was raised in Rabbit using a 6-His tagconjugated to KLH as the immunogen|
Western Blot analysis using His Tag antibody (HRP) (60R-1099)
Detection of His-tagged protein by Western Blot. Samples: 200, 100, or 50 ng of E.coli whole cell lysate expressing a multi-tag fusion protein.Antibodies: Affinity-purified, HRP-conjugated,His Tagantibody 60R-1099 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
|Method of Purification||His Tag antibody (HRP) was purified by affinity chromatography|
|Form & Buffer||Supplied in liquid form in PBS buffercontaining 0.2% BSA and 0.1% Pro-Clean 400|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, WB: 1:1000-1:30,000|
Storage & Safety
|Storage||Stable for 1 year from date of receipt at 2-8 deg C|
|Biological Significance||A His-Tag is a series of at least 6 histidine residues used as a motif at the N- or C-terminus of recombinant proteins. The small size of this tag compared to other common tags, such as GST, makes it less likely to interfere with protein structure or function. This also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media.|
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