His Tag antibody (HRP) (60R-1099)

Rabbit polyclonal His Tag antibody (HRP)

Synonyms Polyclonal His Tag antibody, Anti-His Tag antibody, 6XHis antibody, Polyhistidine Tag antibody, HIS6 Tag antibody, 6-His antibody, HHHHHH antibody, 6-His antibody
Applications ELISA, ICC, WB
Immunogen His Tag antibody was raised in Rabbit using a 6-His tagconjugated to KLH as the immunogen

Images

Western Blot analysis using His Tag antibody (HRP) (60R-1099)

Detection of His-tagged protein by Western Blot. Samples: 200, 100, or 50 ng of E.coli whole cell lysate expressing a multi-tag fusion protein.Antibodies: Affinity-purified, HRP-conjugated,His Tagantibody 60R-1099 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.

Specifications

Host Rabbit
Isotype IgG
Method of Purification His Tag antibody (HRP) was purified by affinity chromatography
Tag/Conjugate HRP
Form & Buffer Supplied in liquid form in PBS buffercontaining 0.2% BSA and 0.1% Pro-Clean 400
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, WB: 1:1000-1:30,000

Storage & Safety

Storage Stable for 1 year from date of receipt at 2-8 deg C

General Information

Biological Significance A His-Tag is a series of at least 6 histidine residues used as a motif at the N- or C-terminus of recombinant proteins. The small size of this tag compared to other common tags, such as GST, makes it less likely to interfere with protein structure or function. This also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media.

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Images

  • Western Blot analysis using His Tag antibody (HRP) (60R-1099) | Detection of His-tagged protein by Western Blot. Samples: 200, 100, or 50 ng of E.coli whole cell lysate expressing a multi-tag fusion protein.Antibodies: Affinity-purified, HRP-conjugated,His Tagantibody 60R-1099 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
  • Western blot analysis using His Tag antibody (HRP) (60R-1099) | Detection of His-tagged protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. His antibody used at 0.04 ug/ml (1:25,000).

Availability: In stock

Price: $225.00
Size: 50 ug
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