HIV1 tat protein (30-AH85)

Purified recombinant HIV1 tat protein

Synonyms HTATIP1 protein, HIV1 tat, Transactivating regulatory protein protein , HIV 1 tat protein, PLIP protein, HIV-1 tat protein, Human tat interactive protein mRNA complete cds protein, HIV-1 tat protein, HIV 1 Tat interacting protein protein, 2interacting protein protein 60 kDa Tat interactive protein protein cPLA protein cPLA2 protein EC 2.3.1.48 protein ESA1 protein Histone acetyltransferase HTATIP protein HIV 1 Tat interacting protein 60kDa protein, HIV 1 tat, TIP60 protein, Tat interactive protein (60kD) protein, protein Tat protein, TIP protein, HIV-1 tat, HIV 1 tat protein
Species Viral
Protein Type Recombinant
Applications ELISA

Specifications

Expression System E.coli
Purity > 99% pure
Method of Purification HIV1 tat protein was purified by Ion affinity and reverse phase HPLC.
Form & Buffer Supplied as a frozen liquid. Dilute tat stock solution in 150mM NaCl, 50mM Sodium Citrate, pH 6.5 immediately before use. Tat readily oxidizes in buffer solutions, which may change its LTR dependent transcriptional activation activity.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user
Bioactivity Tat concentrations in 1 ug/ml range produced at least 25 fold increase in CAT activity. The biological activity of this protein was determined by LTR-CAT activation in scrapie-loaded HeLe Cells, and in U373 MG cells in the presence of 100uM chlorochine di

Storage & Safety

Storage Aliquot and store at -70 deg C or lower. Avoid repeated freeze/thaw cycles

General Information

Biological Significance Human immunodeficiency virus (HIV) is a lentivirus that causes acquired immunodeficiency syndrome (AIDS), a condition in humans in which the immune system begins to fail, leading to life-threatening opportunistic infections. HIV has several major genes coding for structural proteins that are found in all retroviruses, and several nonstructural ("accessory") genes that are unique to HIV.

References
Add a Paper

Elias G. Argyrisa, Joseph Kulkoskya, Marie E. Meyerb, Yan Xua, Muhammad Mukhtara, Roger J. Pomerantza, Kevin Jon Williams

The perlecan heparan sulfate proteoglycan mediates cellular uptake of HIV-1 Tat through a pathway responsible for biological activity

Virology

Volume: 330 Issue: 2 Page: 481-486 DOI: 10.1016/j.virol.2004.10.011

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Availability: In stock

Price: $855.75
Size: 50 ug
OR
Shipping
View Our Distributors