HK1 antibody (10R-2226)

Mouse monoclonal HK1 antibody

Synonyms Monoclonal HK1 antibody, Anti-HK1 antibody, HK 1 antibody, HK1, HK-1, HK 1, HK-1 antibody
Cross Reactivity Human, Rat, Mouse
Applications ELISA, IF, IHC, WB
Immunogen HK1 antibody was raised in Mouse using a purified recombinant fragment of human HK1 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using HK1 antibody (10R-2226) (HK1 antibody)

Immunofluorescence analysis of NIH/3T3 cells using HK1 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Host Mouse
Clone 7A7
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Immunofluorescent staining using Western Blot analysis using HK1 antibody (10R-2226) (HK1 antibody) | Immunofluorescence analysis of NIH/3T3 cells using HK1 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western Blot analysis using HK1 antibody (10R-2226) | Western Blot showing HK1 antibody used against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4) and PC-12 (5) cell lysate.
  • Immunohistochemical staining using HK1 antibody (10R-2226) | Immunohistochemical analysis of paraffin-embedded human salivary gland tissues (left) and kidney tissues (right) using HK1 antibody with DAB staining.

Availability: In stock

Price: $345.00
Size: 100 ul
OR
Shipping
View Our Distributors