HK1 antibody (10R-2231)

Mouse monoclonal HK1 antibody

Synonyms Monoclonal HK1 antibody, Anti-HK1 antibody, HK-1 antibody, HK 1 antibody, Hexokinase 1 antibody, EC 2.7.1.1 antibody, Hexokinase type I antibody, HK I antibody, Brain form hexokinase antibody, Hexokinase-1 antibody, BB404130 antibody, dea antibody, Glycolytic enzyme antibody, HEXOKIN antibody, Hexokinase PI antibody, Hexokinase type I antibody, Tumor isozyme antibody, HK1, HK 1, HK-1, HK 1 antibody, HK-1 antibody
Cross Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC, WB
Immunogen HK1 antibody was raised in Mouse using a purified recombinant fragment of human HK1 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using HK1 antibody (10R-2231) (HK1 antibody)

FC analysis of K562 cells using HK1 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 3A10
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria.

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Images

  • Flow Cytometric assay using Western Blot analysis using HK1 antibody (10R-2231) (HK1 antibody) | FC analysis of K562 cells using HK1 antibody (green) and negative control (purple).
  • Western Blot analysis using HK1 antibody (10R-2231) | Western Blot showing HK1 antibody used against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
  • Immunohistochemical staining using HK1 antibody (10R-2231) | Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 antibody with DAB staining.
  • Immunofluorescent staining using Western Blot analysis using HK1 antibody (10R-2231) (HK1 antibody) | Immunofluorescence analysis of NIH/3T3 cells using HK1 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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