HLF protein (His tag) (80R-1930)
Purified recombinant HLF protein
|Synonyms||MGC33822 protein, Hepatic leukemia factor protein|
|Assay Information||HLF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.|
Coomassie Blue stained SDS-PAGE of HLF protein (His tag) (80R-1930)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-295 amino acids: MGSSHHHHHH SSGLVPRGSH MEKMSRPLPL NPTFIPPPYG VLRSLLENPL KLPLHHEDAF SKDKDKEKKL DDESNSPTVP QSAFLGPTLW DKTLPYDGDT FQLEYMDLEE FLSENGIPPS PSQHDHSPHP PGLQPASSAA PSVMDLSSRA SAPLHPGIPS PNCMQSPIRP GQLLPANRNT PSPIDPDTIQ VPVGYEPDPA DLALSSIPGQ EMFDPRKRKF SEEELKPQPM IKKARKVFIP DDLKDDKYWA RRRKNNMAAK RSRDARRLKE NQIAIRASFL EKENSALRQE VADLRKELGK CKNILAKYEA RHGPL|
|Purity||> 85% pure|
|Molecular Weight||35.3 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 40% glycerol, 0.2M NaCl and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||HLF, also known as hepatic leukemia factor, belongs to the bZIP family and the PAR (proline and acidic-rich) subfamily of transcription regulatory proteins. HLF binds DNA specifically as a homodimer or heterodimer with other PAR factors. It is highly expressed in liver, with lower levels in lung and kidney and functions as a potent trans-activator. Recombinant human HLF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
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