HOXA5 antibody (10R-1521)

Mouse monoclonal HOXA5 antibody

Synonyms Monoclonal HOXA5 antibody, Anti-HOXA5 antibody, HOXA5, MGC9376 antibody, HOXA 5 antibody, HOX1.3 antibody, Homeobox protein Hox-A5 antibody, HOXA 5, HOXA-5 antibody, HOXA-5
Specificity Human HOXA5
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen HOXA5 antibody was raised in mouse using recombinant Human Homeobox A5

Images

Immunofluorescent staining using HOXA5 antibody (10R-1521)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-HOXA5 antibody.

Specifications

Host Mouse
Clone 129C1a
Isotype IgG2a
Method of Purification HOXA5 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer HOXA5 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis.

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Images

  • Immunofluorescent staining using HOXA5 antibody (10R-1521) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-HOXA5 antibody.
  • Western Blot analysis of immunized recombinant protein using HOXA5 antibody (10R-1521)

Availability: In stock

Price: $190.00
Size: 50 ug
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