HPCAL1 protein (His tag) (80R-1478)
Purified recombinant Human HPCAL1 protein
|Synonyms||Calcium-binding protein BDR-1 protein, HPCAL 1, HPCAL1, Hippocalcin-like 1 protein, HPCAL-1 protein, Hippocalcin-like protein 1 protein, visinin-like protein 3 protein, HLP2 protein, HPCAL 1 protein, VILIP3, VILIP-3 protein, BDR1 protein, HPCAL-1|
Coomassie Blue stained SDS-PAGE of HPCAL1 protein (His tag) (80R-1478)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-193 amino acids: MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF|
|Purity||> 95% pure|
|Molecular Weight||24.4 kDa (213aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||HPCAL1, also known as VILIP-3, is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It is involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Recombinant human HPCAL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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