HPRT antibody (10R-1780)

Mouse monoclonal HPRT antibody

Synonyms Monoclonal HPRT antibody, Anti-HPRT antibody, Hypoxanthine guanine phosphoribosyltransferase antibody, HGPRT antibody, HPRT1 antibody, Hypoxanthine phosphoribosyltransferase 1 antibody, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1 antibody, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody, HPRT antibody, HGPRTase antibody
Cross Reactivity Human
Applications ELISA, WB
Immunogen HPRT antibody was raised in mouse using recombinant human HPRT (1-218aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of HPRT antibody (10R-1780)

The extracts of 293T(35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:50

Specifications

Host Mouse
Clone AT2G8
Isotype IgG1, k
Source Anti-human HPRT antibody, clone AT2G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HPRT protein.
Method of Purification By protein-G affinity chromatography
Form & Buffer Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations Each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.

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Images

  • Western Blot analysis of HPRT antibody (10R-1780) | The extracts of 293T(35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:50

Availability: In stock

Price: $270.00
Size: 100 ul
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