HRP antibody (10R-1969)
Mouse monoclonal HRP antibody
|Synonyms||Monoclonal HRP antibody, Anti-HRP antibody, Horseradish Peroxidase antibody|
|Immunogen||HRP antibody was raised in Mouse using a purified recombinant fragment of HRP expressed in E. coli as the immunogen.|
Western Blot analysis using HRP antibody (10R-1969)
Western Blot showing HRP antibody used against full-length HRP recombinant protein.
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Horseradish peroxidase is a 44,173. 9-dalton glycoprotein with 4 lysine residues for conjugation to a labeled molecule. It produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing it to be detected and quantified. HRP is often used in conjugates (molecules that have been joined genetically or chemically) to determine the presence of a molecular target.|
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