HRSP12 protein (His tag) (80R-1597)
Purified recombinant Human HRSP12 protein
|Synonyms||HRSP 12 protein, HRSP 12, HRSP-12, p14.5 protein, HRSP12, Ribonuclease UK114 protein, HRSP-12 protein, UK114, PSP protein|
Coomassie Blue stained SDS-PAGE of HRSP12 protein (His tag) (80R-1597)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-137 amino acids: MGSSHHHHHH SSGLVPRGSH MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL|
|Purity||> 95% pure|
|Molecular Weight||16.6 kDa (157aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||HRSP12, also called ribonuclease UK114, is an endoribonuclease found predominantly in human adult kidney and liver, and which is responsible for inhibiting translation by cleaving mRNA. This protein cleaves phosphodiester bonds only in single-stranded RNA. It may be an important biomarker for heptatic carcinoma. Recombinant human HRSP12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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