Hsp27 antibody (10R-1045)

Mouse monoclonal Hsp27 antibody

Synonyms Monoclonal Hsp27 antibody, Anti-Hsp27 antibody, HS.76067 antibody, CMT2F antibody, HspB1 antibody, Hsp B1 antibody, Heat shock protein beta-1 antibody, Heat shock 27 kDa protein 28 kDa heat shock protein antibody, P25 antibody, Heat shock 28kDa protein 1 antibody, Hsp27 antibody, Heat shock 27 kDa protein antibody, SRP27 antibody, Hsp 27 antibody, Heat shock protein beta 1 antibody, Heat shock 27kDa protein 1 antibody, Heat Shock Protein 27 antibody, Hsp28 antibody, Hsp27 antibody, Estrogen regulated 24 kDa protein antibody, at shock 25kDa protein 1 antibody, Hsp 28 antibody, Hsp27, Hsp -27 antibody, DKFZp586P1322 antibody, Stress responsive protein 27. antibody, HSP25 antibody, Hsp -27, Hsp 27, Hsp25 antibody, Heat Shock Protein27 antibody, HSP 27 antibody, Hspb1 antibody, Growth-related 25 kDa protein antibody, HspB1 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen Hsp27 antibody was raised in mouse using recombinant human Hsp27 (1-205aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of Hsp27 antibody (10R-1045)

Cell lysates of HeLa(each 30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 2A5
Isotype IgG1 kappa
Source Anti-human Hsp27 antibody, clone 2A5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp27 protein.
Method of Purification Hsp27 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:50-1:100, WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPkAP kinase 2 and the stress-activated protein kinase SAPk2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.

References
Add a Paper

James B Whitney, Mark A Wainberg

Impaired RNA incorporation and dimerization in live attenuated leader-variants of SIVmac239

Retrovirology

Issue: 2006, 3:96 DOI: 10.1186/1742-4690-3-96

James B Whitney, Mark A Wainberg

Recovery of fitness of a live attenuated simian immunodeficiency virus through compensation in both the coding and non-coding regions of the viral genome

Retrovirology

Issue: 2007, 4:44 DOI: 10.1186/1742-4690-4-44

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Images

  • Western Blot analysis of Hsp27 antibody (10R-1045) | Cell lysates of HeLa(each 30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $265.00
Size: 100 ul
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