HSP90 protein (His tag) (80R-1003)
Purified recombinant Human HSP90 protein
|Synonyms||HSP 86 protein, FLJ26984 protein, HSPC1 protein, HSP90N protein, hsp90 protein, HSP 90, HSP89A protein, class A member 2 protein, Hsp90 protein, HSP -90, Hsp89 protein, HSPN protein, HSP90ALPHA protein, Renal carcinoma protein NY-REN-38 protein, Heat shock protein HSP 90-alpha, LAP2 protein, D6S182 protein, HSPCA protein, HSPCAL3 protein, Heat shock protein 90-alpha Renal carcinoma protein NY REN 38 protein, FLJ31884 protein, heat shock protein 90kDa alpha (cytosolic) protein, NY REN 38 protein protein, HSP86 protein, HSP90, HSP90AA1 protein, HSPCB protein, Lipopolysaccharide associated protein2 protein, HSP -90 protein, LPS associated protein 2 protein, HSP84 protein, HSP90A protein, HSP 90 protein, Heat shock 90kDa protein 1 alpha protein|
Coomassie Blue stained SDS-PAGE of HSP90 protein (His tag) (80R-1003)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-732 amino acids: MGSSHHHHHH SSGLVPRGSH MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LSSGFSLEDPQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VD|
|Purity||> 90% pure|
|Molecular Weight||86.8 kDa (752aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl, pH 7.4, containing 100 mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||HSP 90 is a human heat shock protein. In response to adverse change in their environment, cells from all organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under nonstress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptors, heme-regulated protein kinase, actin and tubulin. Recombinant human Hsp90 alpha, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.|
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