Human E Selectin ELISA kit (55R-1676)

ELISA kit for the detection of E Selectin in the research laboratory

Synonyms Human E Selectin ELISA kit, CD62E ELISA kit, ELAM-1 ELISA kit, LECAM2 ELISA kit, Selectin E ELISA kit, ESEL ELISA kit
Applications ELISA
Assay Information Sensitivity: 62.5 pg/ml-4000 pg/ml

Images

ELISA assay using Human E Selectin ELISA kit (55R-1676)

Graph showing ELISA absorbance curve (O.D.) of Human E-Selectin (pg/ml)

Specifications

Form & Buffer This Kit contains: Lyophilized recombinant human E-Selectin standard: 10ng/tube

Storage & Safety

Storage Store at 4 deg C for short term storage (4 months) or -20 deg C for long term storage (8 months)

General Information

Biological Significance Endothelial Leukocyte Adhesion Molecule1 (ELAM1, E selectin) belongs to the selectin family of adhesion molecules). Together with LECAM1 (L selectin) and GMP140 (P selectin), E selectin mediates the initial interactions of leukocytes and platelets with endothelial cells. Molecular structure: The extracellular part of all selectins consists of anamino-terminal c-type lectin domain which specifically binds to carbohydrate ligands. This is followed by an EGF-like domain, and, in the case of E-selectin, by 6 short consensus repeats. The transmembrane portion of the molecule is followed by a short cytoplasmic tail. Selectins guide nonactivated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. The potential binding partner of E selectin contains sialyl LewisX oligosaccharide. Other suitable ligands for the lectin domain of E selectin are sialylated, fucosylated lactosaminoglycans. Together with GMP140, E selectin is expressed on cytokine-activated endothelialcells, and contributes to the adhesion of still resting leukocytes to the endothelium. This initial binding event is an essential prerequisite for the activation of the immune cells via different inflammatory mediators. In contrast to GMP140, E selectin is maximally expressed 24 hours after cell activation. Within the next 24-48 hours E selectin is againeliminated from the cytoplasmic membrane by shedding into the circulation. The circulating form or soluble (sE selectin) of this selectin exerts chemotactical signals on neutrophils and additionally activates the 2 integrins sE selectin assists in preparing the migration capacity of these cells.

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Images

  • <div><font face=Calibri size=2>ELISA assay using Human E Selectin ELISA kit (55R-1676)</font></div>     | Graph showing ELISA absorbance curve (O.D.) of Human E-Selectin (pg/ml)

Availability: In stock

Price: $330.00
Size: 1 kit
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