Human IL10 ELISA Kit (55R-1622)

ELISA kit for detection of Human IL10 in the research laboratory

Synonyms Interleukin 10 ELISA Kit, IL-10 ELISA Kit
Applications ELISA
Assay Information Sensitivity: 7.8 pg/ml-500 pg/ml (body fluids, tissue lysates or cell culture supernates), 3.4 pg/ml-250 pg/ml (human sera, plasma)

Images

ELISA assay using Human IL10 ELISA Kit (55R-1622)

Graph showing ELISA absorbance curve (O.D.) of Human IL10 (pg/ml)

Specifications

Form & Buffer This Kit contains: Lyophilized recombinant human IL-10 standard: 10ng/tube

Storage & Safety

Storage Store at 4 deg C for short term storage (4 months) or -20 deg C for long term storage (8 months)

General Information

Biological Significance This ELISA kit for the detection of Human IL10 is a sandwich-type of enzyme immunoassay in which a monoclonal antibody has been precoated to polystyrene microtiter wells. The target is bound by the antibody on the microtiter plate. Non-bound material is then removed by washing. Subsequently, a biotinylated antibody is added. This antibody binds to the cytokine-antibody complex present in the microtiter well. Excess biotinylated antibody is removed by washing, followed by addition of horseradish peroxidase (HRP) conjugated streptavidine, which binds onto the biotinylated side of the cytokine sandwich. After removal of non bound HRP conjugate by washing, substrate solution is added to the wells. A coloured product is formed in proportion to the amount of cytokine present in the sample or standard. After the reaction has been terminated by the addition of a stop solution, absorbance is measured in a microtiter plate reader. From the absorbance of samples and those of a standard curve, the concentration of cytokine can be determined by interpolation with the standard curve.

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Images

  • <div><font face=Calibri size=2>ELISA assay using Human IL10 ELISA Kit (55R-1622)</font></div>     | Graph showing ELISA absorbance curve (O.D.) of Human IL10 (pg/ml)

Availability: In stock

Price: $275.00
Size: 1 kit
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