Human sCD23 ELISA kit (55R-IB49627)

ELISA kit for the detection of sCD23 in the research laboratory

Synonyms Human sCD23 ELISA kit, human soluble CD23 ELISA kit, Human BLAST-2 ELISA kit, Human Lymphocyte IgE receptor ELISA kit
Applications ELISA
Assay Information An antihuman sCD23 coating antibody is adsorbed onto microwells. Human sCD23 present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman sCD23 antibody is added and binds to human sCD23 captured by the first antibody. Following incubation unbound biotin conjugated antihuman sCD23 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman sCD23 antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to`the amount of human sCD23 present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 6 human sCD23 standard dilutions and human sCD23 sample concentration determined.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end-user

Storage & Safety

Storage Store at 2-8 deg C

General Information

Biological Significance CD23 is described as a 45 kDa protein found on the surface of IgM bearing B cells, eosinophils, macrophages and some T and NK cells. It is also found on EBV transformed B cells. Additionally, a released form has been described. When first released the CD23 molecule is 35 kDa; however, this form is quickly cleaved to obtain the more stable, soluble form which is 25 kDa in size. Recently the structure of the CD23 molecule was characterized by cloning and sequencing techniques. Soluble CD23 has been shown to be the B cell growth factor (BCGF). Soluble CD23 is also referred to as Blast2 and as the low affinity IgE receptor (FCeRII). It has been speculated that CD23 may upregulate IgE synthesis in conjunction with T cell promoted interleukin 4; however, the specific physiologic role of this molecule is not yet well understood. Elevated levels of CD23 have been found in research studies of samples from people with B cell-derived Chronic Lymphocytic Leukemia (BCLL), with Hyper IgE Syndrome and post-Bone Marrow Transplantation samples. CD23 levels may be proven to relate to disease course in Hairy Cell Leukemia (HCL).

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Availability: In stock

Price: $805.00
Size: 96 wells
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