Human TNF alpha ELISA kit (55R-1690)

ELISA kit for the detection of TNF alpha in the research laboratory

Synonyms Human TNF alpha ELISA kit, Tumour Necrosis Factor ELISA kit, Cachectin ELISA kit, Cytotoxin ELISA kit, Differentiation-inducing Factor ELISA kit, Necrosin ELISA kit, TNFSF2 ELISA kit
Applications ELISA
Assay Information Sensitivity: 15.6 pg/ml-1000 pg/ml (Body fluids, tissue lysates or cell culture supernates), 7.8 pg/ml-500 pg/ml (Human serum)

Images

ELISA assay using Human TNF alpha ELISA kit (55R-1690)

Graph showing ELISA absorbance curve (O.D.) of Human TNFa (pg/ml)

Specifications

Form & Buffer This Kit contains: Lyophilized recombinant human TNF alpha standard: 10ng/tube

Storage & Safety

Storage Store at 4 deg C for short term storage (4 months) or -20 deg C for long term storage (8 months)

General Information

Biological Significance Tumor Necrosis Factor alpha (TNF alpha), also known as cachectin, is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activatingneutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, aswell as exhibiting tumoricidal activity by inducing localized blood clotting. TNF alpha also inhibits lipoprotein lipaseactivity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF alpha. Due to its varied actions throughout the immune system, TNF alpha may play a role in the pathogenesis of many disease states. TNF alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF alpha within four hours of stimulation by recombinant IL2 and there is some in vitro evidence to suggest that TNF alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GMCSF, and IL1. Recent evidence has suggested an intracellular role for this peptide.

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  • <div><font face=Calibri size=2>ELISA assay using Human TNF alpha ELISA kit (55R-1690)</font></div>     | Graph showing ELISA absorbance curve (O.D.) of Human TNFa (pg/ml)

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Price: $275.00
Size: 1 kit
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