Human VEGF ELISA kit (55R-1700)

ELISA kit for the detection of Human VEGF in the research laboratory

Synonyms Human Vascular Endothelial Growth Factor ELISA kit, VPF ELISA kit, Folliculostellate cell derived growth factor ELISA kit, Glioma derived endothelial cell mitogen ELISA kit
Applications ELISA
Assay Information Sensitivity: 31.2 pg/ml-2000 pg/ml (human sera, body fluids)15.6 pg/ml-1000 pg/ml (cell culture supernates),

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ELISA assay using Human VEGF ELISA kit (55R-1700)

Graph showing ELISA absorbance curve (O.D.) of Human VEGF (pg/ml)

Specifications

Form & Buffer This Kit contains: Lyophilized recombinant human VEGF standard: 10ng/tube

Storage & Safety

Storage Store at 4 deg C for short term storage (4 months) or -20 deg C for long term storage (8 months)

General Information

Biological Significance Vascular Endothelial Cell Growth Factor (VEGF) is a homodimeric protein initially purified from media conditioned by normal bovine pituitary folliculostellate cells and secreted by a variety of vascularized tissues. It was subsequently found to be identical to a vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based upon its ability to increase the permeability of capillary blood vessels. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis and capillary permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains. VEGF is expressed in many human tumor cells, including human adenocarcinoma, human pancreatic carcinoma, human hepatocellular carcinoma, renal cell carcinoma, fibrosarcoma, HL60 promyelocytic leukemia, GS9L glioma and U937 lymphoma cells. In normal tissues, VEGF expression has been observed in activated macrophages, keratinocytes, hepatocytes, smooth muscle cell,s Leydig cells, embryonic fibroblasts and bronchial and choroids plexus epithelium, renal glomerular visceral epithelium and mesangial cells.

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Human VEGF ELISA kit Protocol

Added: Jun 22, 2015

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  • <div><font face=Calibri size=2>ELISA assay using Human VEGF ELISA kit (55R-1700)</font></div>     | Graph showing ELISA absorbance curve (O.D.) of Human VEGF (pg/ml)

Availability: In stock

Price: $330.00
Size: 1 kit
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