HUS1 protein (His tag) (80R-1576)
Purified recombinant Human HUS1 protein
|Synonyms||HUS-1 protein, checkpoint protein HUS1 protein, HUS 1 protein, HUS1, , HUS 1, HUS-1|
Coomassie Blue stained SDS-PAGE of HUS1 protein (His tag) (80R-1576)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-280 amino acids: MGSSHHHHHH SSGLVPRGSH MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS|
|Purity||> 95% pure|
|Molecular Weight||33.8 kDa (300aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 100mM NaCl and 40% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1(RAD9-RAD1-HUS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear protein (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response. Recombinant human HUS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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