ICAD antibody (20R-2910)

Rabbit polyclonal ICAD antibody

Synonyms Polyclonal ICAD antibody, Anti-ICAD antibody, DFFA antibody, DNA fragmentation factor antibody, 45kDa antibody, alpha polypeptide antibody, BOS_15806 antibody, DFF1 antibody, DFF35 antibody, DFF45 antibody, DFF-45 antibody, DNA fragmentation factor 45 kDa subunit antibody, DNA fragmentation factor antibody, alpha polypeptide antibody, DNA fragmentation factor antibody, alpha subunit antibody, DNA fragmentation factor subunit alpha antibody, H13 antibody, ICAD antibody, ICAD-L antibody, ICAD-S antibody, inhibitor of CAD antibody, RP23-121D17.3 antibody
Cross Reactivity Human
Applications WB
Immunogen ICAD antibody was raised in Rabbit using a synthetic target peptide as the immunogen

Images

Western Blot analysis using ICAD antibody (20R-2910)

Specifications

Host Rabbit
Isotype IgG
Method of Purification ICAD antibody was purified by Protein A affinity chromatography
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Usage & Assay Information

Usage Recommendations WB: 1:2000,

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance The inhibitor of caspase-3-activated DNase (ICAD) is a caspase-3 substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of M.W. 45 kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a M.W.35 kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD-specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Western Blot analysis using ICAD antibody (20R-2910) |

Availability: In stock

Price: $295.00
Size: 100 ul
OR
Shipping
View Our Distributors