ID1 antibody (10R-1581)

Mouse monoclonal ID1 antibody

Synonyms Monoclonal ID1 antibody, Anti-ID1 antibody, DNA-binding protein inhibitor ID-1 antibody, ID-1, ID1, ID 1 antibody, ID 1, ID-1 antibody, ID antibody
Specificity Human ID1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen ID1 antibody was raised in mouse using recombinant Human Inhibitor Of Dna Binding 1, Dominant Negative Helixloop-Helix Protein (Id1)

Images

Immunofluorescent staining using ID1 antibody (10R-1581)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ID1 antibody.

Specifications

Host Mouse
Clone 2456C1a
Isotype IgG1
Method of Purification ID1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ID1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

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Images

  • Immunofluorescent staining using ID1 antibody (10R-1581) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ID1 antibody.
  • Western Blot analysis of immunized recombinant protein using ID1 antibody (10R-1581)

Availability: In stock

Price: $190.00
Size: 50 ug
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