ID2 antibody (10R-1478)

Mouse monoclonal ID2 antibody

Synonyms Monoclonal ID2 antibody, Anti-ID2 antibody, GIG8 antibody, ID2H antibody, ID 2, ID2A antibody, inhibitor of DNA binding 2 dominant negative helix-loop-helix protein antibody, MGC26389 antibody, ID-2, ID-2 antibody, ID2, ID 2 antibody
Specificity Human ID2
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen ID2 antibody was raised in mouse using recombinant Human Inhibitor Of Dna Binding 2, Dominant Negative Helix-Loop-Helix Protein (Id2)

Images

Immunofluorescent staining using ID2 antibody (10R-1478)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ID2 antibody.

Specifications

Host Mouse
Clone 2457C5a
Isotype IgG1
Method of Purification ID2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ID2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.

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Images

  • Immunofluorescent staining using ID2 antibody (10R-1478) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ID2 antibody.
  • Western Blot analysis of immunized recombinant protein using ID2 antibody (10R-1478)

Availability: In stock

Price: $190.00
Size: 50 ug
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