IDH3A antibody (70R-1102)

Rabbit polyclonal IDH3A antibody

Synonyms Polyclonal IDH3A antibody, Anti-IDH3A antibody, IDH 3, IDH-3, Isocitrate Dehydrogenase 3 antibody, Nad+ Alpha antibody, IDH-3 antibody, IDH3, IDH 3 antibody
Cross Reactivity Human, Mouse, Rat, Dog
Applications IHC, WB
Immunogen IDH3A antibody was raised using a synthetic peptide corresponding to a region with amino acids MKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLK
Assay Information IDH3A Blocking Peptide, catalog no. 33R-6142, is also available for use as a blocking control in assays to test for specificity of this IDH3A antibody

Images

Western Blot analysis using IDH3A antibody (70R-1102)

IDH3A antibody (70R-1102) used at 1.25 ug/ml to detect target protein.

Specifications

Host Rabbit
Method of Purification Total IgG Protein A purified
Molecular Weight 40 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 100ul distilled water for a 1mg/ml concentration of IDH3A antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1.25 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit.

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Images

  • Western Blot analysis using IDH3A antibody (70R-1102) | IDH3A antibody (70R-1102) used at 1.25 ug/ml to detect target protein.

Availability: In stock

Price: $275.00
Size: 100 ug
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