IDH3G protein (His tag) (80R-2066)

Recombinant human IDH3G protein (His tag)

Synonyms IDH3G, IDHG 3 protein, IDHG 3, IDHG-3 protein, Isocitrate dehydrogenase [NAD] subunit gamma protein, IDHG-3, mitochondrial protein, H-IDHG protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of IDH3G protein (His tag) (80R-2066)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 40-393 amino acids: MGSSHHHHHH SSGLVPRGSH MFSEQTIPPS AKYGGRHTVT MIPGDGIGPE LMLHVKSVFR HACVPVDFEE VHVSSNADEE DIRNAIMAIR RNRVALKGNI ETNHNLPPSH KSRNNILRTS LDLYANVIHC KSLPGVVTRH KDIDILIVRE NTEGEYSSLE HESVAGVVES LKIITKAKSL RIAEYAFKLA QESGRKKVTA VHKANIMKLG DGLFLQCCRE VAARYPQITF ENMIVDNTTM QLVSRPQQFD VMVMPNLYGN IVNNVCAGLV GGPGLVAGAN YGHVYAVFET ATRNTGKSIA NKNIANPTAT LLASCMMLDH LKLHSYATSI RKAVLASMDN ENMHTPDIGG QGTTSEAIQD VIRHIRVING RAVEA
Expression System E.coli
Purity > 85% pure
Molecular Weight 41.1 kDa (375aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT, 2mM EDTA
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance IDH3G, also known as isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, belongs to the isocitrate and isopropylmalate dehydrogenases family. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD(+) as the electron acceptor, and is localised to the mitochondrial matrix. Recombinant human IDH3G protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of IDH3G protein (His tag) (80R-2066) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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