IDI2 protein (His tag) (80R-1758)
Purified recombinant Human IDI2 protein
|Synonyms||IPPI2 protein, IDI2, IDI-2 protein, IDI 2 protein, IDI 2, Isopentenyl-diphosphate delta-isomerase 2 protein, IDI-2|
Coomassie Blue stained SDS-PAGE of IDI2 protein (His tag) (80R-1758)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-227 amino acids: MGSSHHHHHH SSGLVPRGSH MSDINLDWVD RRQLQRLEEM LIVVDENDKV IGADTKRNCH LNENIEKGLL HRAFSVVLFN TKNRILIQQR SDTKVTFPGY FTDSCSSHPL YNPAELEEKD AIGVRRAAQR RLQAELGIPG EQISPEDIVF MTIYHHKAKS DRIWGEHEIC YLLLVRKNVT LNPDPSETKS ILYLSQEELW ELLEREARGE VKVTPWLRTI AERFLYRWWP HLDDVTPFVE LHKIHRV|
|Purity||> 80% pure|
|Molecular Weight||28.9 kDa (247aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||IDI2, also known as isopentenyl-diphosphate delta-isomerase 2, belongs to the IPP isomerase type 1 family. IDI2 catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). Recombinant human IDI2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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